a transferrin receptor 1 cd71 mab  (Thermo Fisher)

Journal: iScience

Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading

doi: 10.1016/j.isci.2025.112273

Figure Lengend Snippet:

Article Snippet: Primary antibodies were used as follows: YAP (1:200; Santa Cruz, sc-101199), GFP (1:2000; Invitrogen, A-11122), PTBP1 (1:500; Thermo Fisher Scientific, 32–4800), Pitx2 (1:1000; Capra Science, PA-1020), CCT2 (1:200; Santa Cruz, sc-374152), Nucleolin (1:300; Santa Cruz, sc-8031), Numb (1:1000; Cell Signaling Technology, 2756), Integrin β-1 (1:1000; Santa Cruz, sc-374429), Transferrin receptor 1 (TfR1) (1:1000; Cell Signaling Technology, 13113), β-tubulin (1:5000; Sigma-Aldrich, T4026), γ-adaptin (1:1000; gift from Dr Andrew Peden, University of Sheffield), GAPDH (1:20000; Proteintech, 60004-1-IG), β-catenin (1:2000; BD Biosciences, 610153).

Techniques: Recombinant, Selection, Staining, Negative Control, Software